 Application Solutions for Purifying and Analyzing PEGylated Proteins Using Various Chromatography Products.
18 Nov 2008 Three application notes for purifying and analyzing PEGylated proteins by reversed phase and gel filtration chromatography are available from Phenomenex®. The application notes feature Phenomenex’s market-leading Jupiter™ and BioSep™ HPLC product lines and provide detailed method development guidance. PEGylation (protein modification using polyethylene glycol) of proteins has become a widespread method for improving the pharmacokinetic properties of protein and peptide therapeutics. Unfortunately, upon modification such protein-PEG conjugates are difficult to purify and analyze using traditional chromatography methods. The research presented in the application notes uses different PEG modifiers to look at the effect such modifiers had on the chromatography of several different proteins and peptides. Separation methods were optimized fo...
|
|
